Rabbit experimental technical service operation
Today's technical information for your specific analysis of the operation of rabbit experimental technical services: together with Xiaobian to understand it!
1. Experimental technical service materials: The rabbits were quickly killed by cervical dislocation. Then, the whole animal was immersed in a beaker containing 75% ethanol for several seconds, and then taken out and placed in a large dish to carry a clean bench. Use sterile scissors to cut the skin after re-sterilization with iodine and ethanol, and remove the liver or kidney by laparotomy. Place in a sterile dish.
2. Experimental technical service cutting: Wash the removed organs three times with sterile PBS solution, then carefully cut the tissue repeatedly with ophthalmic surgical scissors until it is a small piece of about 1mm3, then wash it with PBS, wash it to the tissue block and whitish until. Transfer to a sterile centrifuge tube and let stand for a few minutes to allow the tissue block to naturally settle to the bottom of the tube and discard the supernatant.
3. Experimental technical service digestion, inoculation culture: Pipette 1 ml of 0.25% trypsin-0.02% EDTA mixed digestive juice, add to the centrifuge tube, mix with the tissue block, add a tube plug, and digest for 8 to 10 minutes in a 37 ° C water bath. Shake the test tube every few minutes, make the tissue and digestive juice fully contact, stand still, aspirate the supernatant, add 5-10 ml of MEM medium containing 5% calf serum to the centrifuge tube, mix and mix with a pipette, and transfer to the second. In a culture flask, it is placed in a carbon dioxide incubator for cultivation.
Dear Customer:
Hello, our company is a high-quality development team with strong technical force, providing high-quality products, complete solutions and high-quality technical service companies for the majority of users. The main products are experimental technical services,
Wuhan Cell Bank
,
Basic research of experimental subjects
Wait.
The company adheres to the tenet of establishing business with integrity, keeping the quality and keeping the industry, and embarking on the goal of making progress. It will continue to climb new peaks at a firmer pace and contribute to the national automation industry. Welcome new and old customers to purchase their favorite products with confidence. We will be happy to help you!
Fork Type Terminals,Insulated Bullet Sockets Terminals,Insulated Bullet Terminals,Type Fork Insulate Terminal
Taixing Longyi Terminals Co.,Ltd. , https://www.longyiterminals.com