1. Defining which protein to detect;
2. Identify which genes encode the protein have the best homology with other species;
3. Find kits for detecting proteins of these species;
4. Consult the supplier ELISA is the type of antibody used in the kit: polyclonal or monoclonal antibody, which antigenic determinant is targeted;
5. Make your own judgment which kit to buy.
Key points:
1. Store according to the label instructions and return to room temperature before use. Standards after dilution should be discarded and cannot be stored.
2. The slats not used in the experiment should be immediately put back into the packaging bag and sealed to prevent deterioration.
3. Do not mix reagents of different batch numbers and use them before quality assurance.
4. Avoid opening the lid for a long time, and read the OD value immediately after the experiment is completed.
5. The order of addition of reagents should be consistent to ensure that all wells are incubated for the same time.
6. Perform the incubation according to the time indicated in the instructions, the amount of liquid added, and the sequence.
Shanghai Jinma Experimental Equipment Co., Ltd. supplies high-quality ELISA test kits. Advantages: high accuracy, high sensitivity, high specificity, short detection time, easy operation, safe and reliable. Tax-included shipping, free agent and technical guidance. For more technical information, please call us.
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